Stock solution recipies: tris-hcl buffer - ...

Dissolve the required mass of tris into a volume of deionized water approximately 1/3 of the desired volume of buffer to be made. 4) adjust the ph

Western , page 1 of 4 western blot protocol

Western, page 1 of 4 western blot protocol (mark hickman, february 2009) growth and protein extraction 1. you want ~25ml of mid-log cells (2 x 107 cells/ml or od

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