Stock solution recipies: tris-hcl buffer - ...
Dissolve the required mass of tris into a volume of deionized water approximately 1/3 of the desired volume of buffer to be made. 4) adjust the ph
Western , page 1 of 4 western blot protocol
Western, page 1 of 4 western blot protocol (mark hickman, february 2009) growth and protein extraction 1. you want ~25ml of mid-log cells (2 x 107 cells/ml or od