Dispase ii (neutral protease, grade ii) - 登录和密码 ...

Www.roche-applied-science.com dispase ii (neutral protease, grade ii) 1. what this product does contents 5 bottles dispase ii lyophilizate, non-sterile,5×1g

Safety data sheet

Safety data sheet according to ec directive 1907/2006/ec dispase ii (neutral protease, grade ii) version 1.2 page 1/4 processing date 23.03.2010 printing date 20...

Iii.h) dispase treatment to disperse cells

Dispase ii (roche cat#10165859001, lot# 933577201). the units of enzymatic activity per mg was not given on the bottle, so i contacted roche for this information and

Adult primary mouse satellite cell culture

Dispase type ii roche 4942078001 5x1g cacl 2 sigma c1016-100g 100g ham's f-10 invitrogen 11550-043 500ml dmem-low glucose cellgro/fisher 10-014-cm 500ml...

Ii. reagents, solutions, and media

Dispase ii roche, cat #101658590001 poly-l-lysine sigma, p1399 complete media 210ml rpmi 1640 25ml horse serum 12.5ml fbs 2.5ml p/s/g (100x) 250ml mpc...

Impact of cell source on human cornea reconstructed by tissue ...

Was incubated in a 2 mg/ml dispase (dispase ii; roche diagnostic, laval, qc, canada) in hepes buffer (ph 7.4) for 18 hours at 4°c. the

Chapter two

Two suitable stem cell source for cartilage tissue engineering abstract the ability of stem cells to self-renew as well as their multilineage differentiation potential

Protease-activated receptors 1 and 4 mediate thrombin signaling in ...

And dispase i (0.125-0.25 mg/ml) or dispase ii (roche) (3-5 mg/ml) at 37°c for 60 minutes with shaking. digested tissue was dispersed by

B. myoblast recipes - university of rochester medical center ...

Dispase ii boehringer mannheim/roche #295 825, aliquot and store at -20 o c. primary culture media (to establish line) 50% maintenance media without bfgf, (below)

A novel method of isolation, preservation, and expansion of human ...

Dispase ii and collagenase a were obtained from roche (indianapolis, in). hydrocortisone, di... grate after dispase ii digestion (data not shown). notably, after

Gene expression & protein expression reagents

For more information, please visit www.roche-applied-science.com/sis/ geneexpression/index.jsp. .. dispase ii (neutral protease, grade ii)...

Mastcellsarecrucialforearlyinflammation ...

(dispase ii; roche, mannheim, germany).after incubation, epidermis was peeled off and fixed in acetone. lcs were stained with a mab against mhc

Supplemental material detailed methods

Collagenase b (roche), 2.4u/ml dispase ii (roche) and 2.5mmol/l cacl2. mononuclear cells were stained with hoechst 33342 (sigma cat #b2261)...

Icos-ligand, expressed on human endothelial cells, costimulates ...

Were isolated from umbilical cords with 2.4 units ml dispase ii (roche molecular biochemicals) and cultured in endothelial growth medium with ec growth supplement...

Supplementary figure legends fig. s1: nfatc2 deficiency is ...

... and 50 u/ml dispase ii (roche). digestion was performed by. 4 incubating the pieces at 37°c for 20 minutes. after 20 minutes, the solution was passed...

Regulation of myosin heavy chain expression during rat skeletal ...

Molecular biology of the cell vol. 12, 1499-1508, may 2001 regulation of myosin heavy chain expression during rat skeletal muscle development in vitro

Reciprocal regulation of cxcr4 and cxcr7 in intestinal mucosal ...

Mg/ml dispase ii (roche, mannheim, germany) in rpmi at 37°c. the mucosa was then incubated with 1 mg/ml collagenase (sigma-aldrich)/50

Neurobiologyofdisease activity ...

Lagenase/dispase ii (roche) for2hatroom temperature. after a wash in 1 pbs, cells were triturated in schneider's drosophila medium (invit-

In vitro cmv-infection model in fresh and glycerolized skin graft

Status, were incubated with 2 mg/ml dispase ii (roche manheim, germany) o/n at 4°c. after in-cubation, dermal and epidermal layers were me-chanically separated.

Presynaptic (type iii) cells in mouse taste buds sense sour (acid ...

Collagenase a (roche), 2.5 mg ml−1 dispase ii (roche), and 1mgml−1 trypsin inhibitor (sigma) directly under the epithelium surrounding taste papillae. the peeled

Supplementary information immunomodulatory properties of stem ...

(worthington biochemicals corp, lakewood, nj) and 0.1% dispase ii (roche diagnostic/boehringer mannheim corp., indianapolis, in) in phosphate buffered saline...

An evi1-c/ebp complex controls peroxisome proliferator-activated ...

... (ii)directlyinducingtheexpre ssionof adiponectin, a secreted factor that enhances insulin action in muscle and liver (18, 23), and (iii) promoting the...

A5a primary rat olfactory receptor neuron culture

Dispase ii, 165859, roche dnase i, d4513, sigma fungizone, 152900187, invitrogen gentamycin, g3632, sigma hepes (hemi) salt, h7637, sigma hyaluronidase...

Rorγ-expressing th17 cells induce murine chronic intestinal ...

Germany), and dispase ii (roche diagnostics). digested tissue was passed through a 40- m cell strainer, and the remaining cellular content was separated from debris

Intracellular ca depolarization produce atp secretion from taste ...

1mgml−1 collagenase a (roche), 2.5mgml−1 dispase ii (roche)and1mgml−1 trypsininhibitor(sigma,st louis, mo, usa) directly under the epithelium surrounding...

Supporting information

Dispase ii (roche) at 4 °c. the epidermis was then separated from the dermis using forceps and further digested into single suspended keratinocytes with tryple...

Sop:propagationofhumanolfactory neurosphere;derivedcells ...

Dispase ii roche 10295825001 12951100 2.4u/ml tryple gibco 12604 527167 786710 poly-l-lysine sigma p6282 107k5107 h 2o gibco 10977-015 527182 egf...

Neurobiologyofdisease ...

Papain (worthington), 1 u/ml dispase ii (roche diagnostics), and 250 u/ml dnase i (worthington)]... (roche diagnostics) in a t75 flask with 12 ml of 50% dmem

Chapter 4

Dispase ii (roche applied science, indianapolis, in). store 4°c. 8. dulbecco's modified eagle medium/nutrient mix f-12 (dmem/f-12) with hepes and l-glutamine...


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